We present an effective and scalable protocol for lentivirus production and transduction of cell lines that is applicable to small numbers (e.g. 25 000 cells) as well as high numbers of cells …

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

May 1, 2025 · Lentiviruses (a genus of retrovirus) express reverse transcriptase, which converts the viral RNA to double stranded DNA, and integrase, which inserts this viral DNA into the host …

The LipExoGen Lentiviral Expression SystemTM uses a third-generation, replication-incompetent, VSV-G-pseudotyped HIV-1-based vector for efficient transduction of dividing and non-dividing …

Dec 25, 2024 · Discover how lentiviral transduction enables stable gene expression in research and therapy. Learn about its applications, benefits, and experimental procedures.

This protocol provides a step-by-step guide on how to conduct lentiviral transduction for the efficient delivery of transgenes. Note: Performing Lentiviral experiments may require special …

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Though replication-deficient, recombinant lentivirus transduces mammalian cells and should be handled with BSL-2 standards. The following protocol is a general method for transducing …

Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and nondividing cells. The addition of puromycin will allow you to select cells that are stably …

Mar 21, 2016 · Lentiviral transduction is an efficient method for the delivery of transgenes to mammalian cells and unifies the ease of use and speed of transient transfection with the …